Method for staining both acid-fast and chromophobic tubercle bacilli with carbolfuschsin.
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چکیده
منابع مشابه
Studies on the effect of starvation on mycobacteria.
Ten cultures of Mycobacterium tuberculosis, one of Mycobacterium kansasii (nonsignificant), and one of Mycobacterium phlei were submitted to starvation. As a result they lost first their acid fastness and then all other staining affinities but, in this chromophobic state, they survived for at least 2 years and, after that time, produced cultures of acid-fast bacilli when transferred onto nutrie...
متن کاملDual Nature of Acid-Fastness **
Acid-fastness is usually defined by the Ziehl-Neelsen technique: staining in carbol-fuchsin, differentiation in HCl-alcohol, and counterstaining in methylene blue.8 As a result, acid-fast substances are red, non-acid-fast materials, blue. Some authors have felt that this property of differential staining resided in one specific type of compound, the mycolic acid type of long-chain fatty acid fo...
متن کاملSome Artifacts Encountered in Stained Preparations of Tubercle Bacilli: I. Non-Acid-fast Forms Arising From Mechanical Treatment.
The first report in this series presented evidence to show that artifacts may arise from the mechanical manipulation of tubercle bacilli while they are being spread on a slide for staining (Yegian and Porter, 1944). During such treatment the normally acid-fast cells can be transformed into the minute non-acidfast rods and granules that have been frequently interpreted as immature forms in a rep...
متن کاملProcedure for demonstrating lepra bacilli in paraffin sections.
The lesser degree of acid-fastness of lepra bacilli as compared with tubercle bacilli appears in a disagreeable manner in the difficulty of demonstrating the organisms of leprosy in paraffinized tissues (1). Faraco (2) showed that by ordinary methods of demonstrating acid-fast organisms the lepra bacilli are often not acid-fast, are not differentiated and may be stained by the counterstain. He ...
متن کاملComparitive Evaluation of Various Staining Techniques for Identification of Tubercle Bacilli by Direct Microscopy
The aim of this study was to compare Fluorescent(auraminO rhodamine,AO)method ,Ziehl-Neelsen (ZN hot method)and kinyoun(cold method) staining techniques for the diagnosis of Mycobacterium tuberculosis. Materials And Methods: 186(on 3 consecutive days) sputum samples were collected from 62 suspected pulmonary tuberculosis patients, processed by petroffs method, and subjected to direct microscopy...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 93 4 شماره
صفحات -
تاریخ انتشار 1967